Cloning our gene of interest to add a green tag
Aim: For our experiments we want to clone the collagen gene into a DNA plasmid that contains genes for green and red fluorescent proteins, which we will then insert into some mammalian cells. Last week I cloned the collagen gene into the DNA plasmid but I have mixed up my tubes. I now need to identify which tube contains the correct plasmid.
Method: I will use three restriction enzymes to cut each of the possible plasmids. I will then check the DNA fragments to identify the correct plasmid.