Cloning Puzzle

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Clue 1

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Clue 2

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Answer

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DNA vector 1: p.gene of interest_Amp

Size: 6000bp

DNA Vector 2: p.gene of interest-GFP_Amp_RFP

Size: 7000bp

Figure 1: My two DNA vectors. One has the green tag on at the end of the XX gene and the other doesn’t.

That’s what the correct plasmid should look like!

Figure 2: Agarose gel showing fragments of DNA after enzyme digestion from each tube (1-6).

After running multiple reactions to make other vector constructs as well I run a gel after digesting all 6 constructs with enzymes 1-3. Tube contains DNA Vector 2: p.gene of interest-GFP_Amp_RFP. This can now be put in the cells.

Which tube contained the right final vector we wanted (DNA vector 2: p.gene of interest-GFP_Amp_RFP)?