Aim: We have put our collagen plasmid into some mammalian cells. Now I need to check that my plasmid is in the cells by looking for the presence of the fluorescent proteins. Green fluorescent protein shines green and red fluorescent protein shines red when excited using the right wavelength of light.
I can use two methods to look at the protein levels in my cells.
To excite the cells means to give them energy by shining a laser at them (not to show them a fun time)